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kapa hyper prep kit  (Roche)


Bioz Manufacturer Symbol Roche manufactures this product  
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    Structured Review

    Roche kapa hyper prep kit
    Kapa Hyper Prep Kit, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/kapa+hyper+prep+kit/HyperPrep/pmc13112862-16-8-12
    Average 99 stars, based on 5 article reviews
    kapa hyper prep kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Construct:

    Article Title: The complete mitochondrial genome of Bionychiurus tamilensis (Collembola: Onychiuridae)
    Article Snippet: .. A genomic DNA library was constructed using the KAPA Hyper Prep Kit (Kapa Biosystems, Woburn, MA) and sequenced with paired-end reads on the Illumina Nextseq1000 platform (Illumina Inc., San Diego, CA). .. The mitogenome was assembled using a two-step process in Geneious Prime v.2025.0.3 (Biomatters Ltd, Auckland, New Zealand).

    Article Title: Retinoic acid resolves the conflict between X-chromosome inactivation and pluripotency program in female cleavage-stage embryos
    Article Snippet: .. After amplification, cDNA 3’ terminal was labeled by biotin and then fragmented. cDNA library was constructed by KAPA Hyper Prep Kit (KK8504, KAPA Biosystems, USA). .. Sequencing was performed on Illumina HiSeq 4000.

    Sequencing:

    Article Title: Hepatic-Differentiated Subpopulation in Clear Cell Renal Cell Carcinoma: A Multi-Omics Analysis of Tumors With Lymphovascular Invasion.
    Article Snippet: .. Whole- genome sequencing was performed using the PCR- free KAPA Hyper Prep Kit (Roche Diagnostics, Basel, Switzerland) with an average coverage of ≥ 60× for tumor samples and ≥ 30× for normal samples. .. RNA sequencing libraries were prepared after ribosomal RNA depletion using the Ribo Zero Kit (Illumina, San Diego, CA, USA) and the TruSeq Stranded Total RNA Kit (Illumina, San Diego, CA, USA), achieving ≥ 100 million total reads with ≥ 20 million reads mapping to mRNAs.

    Article Title: Mycobacterium tuberculosis long-chain fatty acid resistome reveals universal stress protein TB15.3 as essential for infection.
    Article Snippet: .. Briefly, genomic DNA (150-200 ng) was sheared and HiSeq sequencing libraries were prepared using the KAPA Hyper Prep Kit (Roche). ..

    Polymerase Chain Reaction:

    Article Title: Hepatic-Differentiated Subpopulation in Clear Cell Renal Cell Carcinoma: A Multi-Omics Analysis of Tumors With Lymphovascular Invasion.
    Article Snippet: .. Whole- genome sequencing was performed using the PCR- free KAPA Hyper Prep Kit (Roche Diagnostics, Basel, Switzerland) with an average coverage of ≥ 60× for tumor samples and ≥ 30× for normal samples. .. RNA sequencing libraries were prepared after ribosomal RNA depletion using the Ribo Zero Kit (Illumina, San Diego, CA, USA) and the TruSeq Stranded Total RNA Kit (Illumina, San Diego, CA, USA), achieving ≥ 100 million total reads with ≥ 20 million reads mapping to mRNAs.

    Article Title: Integrative fragmentomic and mutational signature profile of plasma cfDNA for early lung cancer detection.
    Article Snippet: The concentration of the extracted cfDNA was subsequently measured using the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, USA). .. The PCR-free WGS library construction was automated using the Biomek (Beckman Coulter, USA) and involved 5-10 ng of the cfDNA sample along with the KAPA Hyper Prep Kit (KAPA Biosystems, USA). .. Constructed libraries were quantified using the KAPA SYBR FAST AR TI CL E IN P RE SS qPCR Master Mix (KAPA Biosystems, USA) and sequenced using paired-end reads on NovaSeq platforms (Illumina, USA).

    Whole Complete genome sequencing:

    Article Title: Integrative fragmentomic and mutational signature profile of plasma cfDNA for early lung cancer detection.
    Article Snippet: The concentration of the extracted cfDNA was subsequently measured using the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, USA). .. The PCR-free WGS library construction was automated using the Biomek (Beckman Coulter, USA) and involved 5-10 ng of the cfDNA sample along with the KAPA Hyper Prep Kit (KAPA Biosystems, USA). .. Constructed libraries were quantified using the KAPA SYBR FAST AR TI CL E IN P RE SS qPCR Master Mix (KAPA Biosystems, USA) and sequenced using paired-end reads on NovaSeq platforms (Illumina, USA).

    Amplification:

    Article Title: Retinoic acid resolves the conflict between X-chromosome inactivation and pluripotency program in female cleavage-stage embryos
    Article Snippet: .. After amplification, cDNA 3’ terminal was labeled by biotin and then fragmented. cDNA library was constructed by KAPA Hyper Prep Kit (KK8504, KAPA Biosystems, USA). .. Sequencing was performed on Illumina HiSeq 4000.

    Labeling:

    Article Title: Retinoic acid resolves the conflict between X-chromosome inactivation and pluripotency program in female cleavage-stage embryos
    Article Snippet: .. After amplification, cDNA 3’ terminal was labeled by biotin and then fragmented. cDNA library was constructed by KAPA Hyper Prep Kit (KK8504, KAPA Biosystems, USA). .. Sequencing was performed on Illumina HiSeq 4000.

    cDNA Library Assay:

    Article Title: Retinoic acid resolves the conflict between X-chromosome inactivation and pluripotency program in female cleavage-stage embryos
    Article Snippet: .. After amplification, cDNA 3’ terminal was labeled by biotin and then fragmented. cDNA library was constructed by KAPA Hyper Prep Kit (KK8504, KAPA Biosystems, USA). .. Sequencing was performed on Illumina HiSeq 4000.



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